Work-related musculoskeletal disorders (WRMSDs) is an umbrella term for symptoms caused or worsened by work. Musculoskeletal disorders have been considered as an impactful occupational problem among most working categories. Our data could be used in future studies as a reference to assess the risk of WRMSDs in other health-care professionals’ population. Further studies should be addressed to identify specific interventions aimed at reducing the burden of WRMSDs including ergonomic education and physical rehabilitation. Perioperative nurses, in general, are steadily exposed to both physical and temporal risk factors. WRMSDs represent a high prevalence issue among perioperative nurses. Meta-regression showed that sex, age, and BMI were not significant predictors of low-back disorders ( p = 0.69 R 2 = 0). Twenty-two studies, considering 3590 perioperative nurses, were included in the systematic review. The contribution of perioperative nurses’ sex, age, and BMI was assessed through a meta-regression. A meta-analysis calculating event rates, and relative 95% Confidence Intervals (CI) was performed for each musculoskeletal body region. Medline, Cumulative Index to Nursing and Allied Health Literature, Scopus, Web of Science, Cochrane Library and Joanna Briggs Institute Database were systematically searched. To evaluate the prevalence of work-related musculoskeletal disorders (WRMSDs) in perioperative nurses and to explore their association with personal characteristics.